Title of the Course : Microbiology & immunology
Nature of course : Minor VI
Code : BOT-MIN-06
Total Credits : 04
Distribution of Marks : 100 [End: 60 (Theory: 45, Pract: 15), In: 40] Total
Course outcomes:
1) To classify microorganism based on different parameters
2) To demonstrate different processes involved in microbiology
3) To differentiate between microbial groups
4) To apply microbes for human welfare
5) to understand the basic immunological processes
6) to evaluate different antibiotics and vaccines
Learning Outcome:
1) To distinguish microbes based on different parameters
2) To understand different microbial processes for application in human welfare 3) To describe immunological concepts
4) To understand antibiotic classes and vaccines
Unit I: Brief history and development of microbiology: (10 classes)
Introduction to study of Microbiology, conflict over spontaneous generation, role of microorganisms in disease, scope of microbiology, development of Koch’s postulate. Classification of microorganisms; morphological, biochemical and molecular characteristics; nutritional types in microorganisms; Culture media, microbial growth curve uncultured microbes.
Unit II: Prokaryotic cell structure: (07 classes)
Bacterial cell wall, cytoplasmic structure and inclusions bodies, sporulation and spore, diversity in bacterial structure; actinomycetes, rickettsias, mycoplasma; archaea.
Unit III: Viruses: (08 classes)
Basic structures, classification, double-stranded and single-stranded DNA and RNA viruses, replication strategies of DNA and RNA viruses; viroids and prions; bacteriphages with suitable examples.
Unit-IV: Application of microbes: (08 classes)
Role of microbes in bio-geo-chenical cycling, Biological nitrogen fixation; Industrial application of microbes: alcohol, organic acids, vaccine, antibiotics; microbial biofilm; wastewater treatment; bifertilizer and biopesticides; Microbial diseases and their control: Host pathogen relationship, mechanisms of virulence, quorum sensing, pathogenesis in plants and animals
Unit-V: Immunology: (10 classes)
Immune response, discrimination between self and non-self, innate and acquired immune response; Innate Immunity: Anatomic and Physiological Barriers, Inflammation, Toll receptors and PAMPs, DAMPs , Defensisns, and Complement system, NK Cells; Antigen: Antigenicity vs immunogenicity, B and T cell epitopes; Immunoglobulins: Basic structure, Ig fold and domains, Classes and subclasses of Ig, Biological activities of Igs, B cell receptor; MHC and Antigen Presentation; Cytokines and cytokine receptors; autoimmunity.
Lab activities
1. Bacterial growth on solid and broth media, pure culture technique, slant preparation 2. Bacterial colony morphology and diversity
3. Gram staining of bacteria
4. Biochemical characterization of bacteria
5. Determination of coliform group in water samples by presumptive, confirmed and completed test.
6. Study of cells involved in immunology (Photograph/animation)
Textbooks
1. Willey, J., Sherwood, L. and WoolvertonC., Microbiology, 10th edition (McGraw-Hill Science, 2017).
2. Pelczar, M.J., Chan, E.C.S. and Kreig, N.R., Microbiology, Publisher McGraw Hill Education (India) Private Limited, ISBN-10 0074623206, 5th Edition, 2001.
3. Tortora, G.J., Fernke, B.R. and Case, C.L., Microbiology – An Introduction, 9th Edition,
4. Basic Immunology: Functions and Disorders of the Immune System, Abul K. Abbas, Andrew H. Lichtman, Shiv Pillai ( Elseviers Saunders 4 th Edition).
5. Kuby Immunology, Thomas J. Kindt, Barbara A. Osborne, Richard A . Goldsby (W.H. Freeman Publishers, Sixth Edition).
Suggested Readings
1. M. T. Madigan, J. M. Martinko, K. S. Bender, D. H. Buckley, D. A. Stahl, T. Brock, Brock Biology of Microorganisms, 14th Edition , Pearson Hall International, 2017.
2. Molecular and Cell Biology (Schaum’s Outlines series special Indian edition) by W. D. Stansfield, J. S.C. Colome, R. J. Cano and R. N. Sharan (2010), McGraw Hill Education
3. Delves, P., Martin, S., Burton, D., Roitt, I. Roitt's Essential Immunology (Wiley Blackwell, 11th Edition).
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