SEM VII (Minor)

 

Title of the Course : Advanced analytical techniques 

Nature of course : Minor 

Code : BOT-MIN-07 

Total Credits : 04 Distribution of Marks : 100 [End: 60 (Theory: 45, Pract: 15), In: 40] 

Total classes : 60

Unit-I : 11 classes

 Nucleic acid extraction and visualization, Agarose Gel Electrophoresis. Polymerase Chain Reaction (PCR)- principle and types, Plasmid isolation and purification 

Unit II: 12 classes 

Bioimaging techniques- electron microscopy principle, uses and sample preparation, fluorescent microscopy principle and uses, Flow cytometry – principle and application, Fluorescence in-situ hybridization, Genomic in-situ hybridization and Fluorescence-Activated Cell Sorting (FACS)

Unit III: 13 classes 

Biophysical Methods: High Performance Liquid Chromatography (HPLC), Fourier Transform Infrared Spectroscopy (FTIR), Nuclear Magnetic Resonance Spectroscopy (NMR), X-ray diffraction (XRD), Gas chromatography (GC), Mass Spectroscopy (MS) 

Unit IV: 09 classes 

DNA sequencing techniques- Pyrosequencing, Nextgen sequencing, Genome editing – CRISPR

Lab activities 

1. Nucleic acid extraction from biological samples 

2. Visualization of DNA by Agarose gel electrophoresis 

3. Nucleic acid amplification by PCR 

4. Demonstration of HPLC, FTIR, NMR, XRD, etc. 

Suggested Readings:

1.Principles of Biochemistry and Molecular Biology by K. Wilson and J. Walker, Cambridge University Press. 

2.Principles of Instrumental analysis by D. A. Skoog and J. J. Leary, Saunders College Publishing, Philadelphia. 

3.Textbook of Structural biology by Anders Lilgas, Lars Lilgas, JuiPiskur et al, World Scientific Publisher